[Features of thiamine metabolism with vitamin B1 deficiency in hypokinesis]

Abstract

The authors have studied activity of thiamine-dependent enzymes: transketolase, pyruvate- and alpha-ketoglutarate dehydrogenases, the content of thiamine diphosphate in the tissue of the liver and musculus gastrocnemius, activity of transketolase of the whole blood hemolysates and different age populations of red blood cells under 15-day hypokinesia with B1-deficiency induced by enteral administration of oxythiamine. The animals received oxythiamine in a dose of 2 mg/kg during 15 days. It was found that limitation of motor activity significantly aggravated the course of vitamin deficiency resulting in disordered utilization of coenzymic thiamine, and in a more pronounced suppression of vitamin-dependent enzymes (transketolase, alpha-ketoglutarate dehydrogenase) activity.