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. 2017 May;27(3):939-952.
doi: 10.1007/s10895-017-2029-y. Epub 2017 Feb 3.

Synthesis, Characterization and Luminescence Sensitivity with Variance in pH, DNA and BSA Binding Studies of Ru(II) Polypyridyl Complexes

Ravi Kumar Vuradi  1 , Srishailam Avudoddi  1 , Venkat Reddy Putta  1 , Laxma Reddy Kotha  1 , Praveen Kumar Yata  1 , Satyanarayana Sirasani  2
Affiliations

Synthesis, Characterization and Luminescence Sensitivity with Variance in pH, DNA and BSA Binding Studies of Ru(II) Polypyridyl Complexes

Ravi Kumar Vuradi et al. J Fluoresc. 2017 May.

Abstract

Three ruthenium(II) polypyridyl complexes, [Ru(phen)2(mip)](ClO4)2 (1) (phen =1,10-Phenanthroline), [Ru(bpy)2(mip)](ClO4)2 (2) (bpy = 2,2'bipyridyl) and [Ru(dmb)2(mip)](ClO4)2 (3) (dmb = 4, 4'-dimethyl 2, 2'-bipyridine), were synthesized with an intercalative ligand mip (2-morpholino-1H-imidazo[4,5-f][1, 10]phenanthroline) and characterized by 1H, 13C-NMR, IR, UV-vis, mass spectra and elemental analysis. pH effect, ion selectivity (cations, anions) and solvent sensitivity of complexes were studied. The interaction of these complexes with DNA was performed using absorption, emission spectroscopy and viscosity measurements. The experimental results indicated that the two complexes interacted with calf thymus DNA (CT-DNA) by intercalative mode. BSA (Bovine Serum Albumin) protein binding of these complexes was studied by UV-visible and fluorescence techniques. The binding capacity of these complexes was explained theoretically by molecular docking method.

Keywords: BSA binding; DNA binding; Docking studies; Ru(II) complexes; Sensor effect; pH effect.

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