Draft genome sequences of eight bacteria isolated from the indoor environment: Staphylococcus capitis strain H36, S. capitis strain H65, S. cohnii strain H62, S. hominis strain H69, Microbacterium sp. strain H83, Mycobacterium iranicum strain H39, Plantibacter sp. strain H53, and Pseudomonas oryzihabitans strain H72

Abstract

We report here the draft genome sequences of eight bacterial strains of the genera Staphylococcus, Microbacterium, Mycobacterium, Plantibacter, and Pseudomonas. These isolates were obtained from aerosol sampling of bathrooms of five residences in the San Francisco Bay area. Taxonomic classifications as well as the genome sequence and gene annotation of the isolates are described. As part of the "Built Environment Reference Genome" project, these isolates and associated genome data provide valuable resources for studying the microbiology of the built environment.

Keywords: Airborne bacteria; Bacterial genomes; Built environment; Shower water.

Fig. 1

Maximum Likelihood tree based on concatenated markers from Staphylococcus spp. genomes. The tree was inferred using FastTree from an Hmmalign alignment in Phylosift of 37 highly conserved marker genes. Numbers at the nodes represent local support values. The tree was rooted to Macrococcus caseolyticus as an outgroup (not shown) since this species is a close relative to Staphylococcus

Fig. 2

Transmitted light microscope images of the eight isolates. Bar is 5 μm. a Rod-shaped cells of Microbacterium sp. H83 b Mycobacterium iranicum H39; note, this organism was sparse in the images and tended to be highly clumped, so two snapshots were used for the sake of visualization c pleomorphic, rod-shaped cells of Plantibacter sp. H53 d Pseudomonas oryzihabitans H72, rods with rounded ends typically occurring as solitary cells but rarely also in pairs, e Staphylococcus capitis H36, occurring in pairs or strings of cells f Staphylococcus capitis H65, as single cells and pairs g Staphylococcus cohnii H62, as single cells, pairs, and occasionally threes or tetrads, h Staphylococcus hominis H69, as single cells and pairs. Images were collected using a Zeiss M1 AxioImager equipped with DIC and a Hamamatsu Orca 03 camera run by BioVision’s iVision software. Images were cropped and organized into a plate using Adobe Photoshop CS6