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. 2017 Sep;64(5):716-720.
doi: 10.1111/jeu.12399. Epub 2017 Mar 8.

Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China

Lei Deng  1 , Wei Li  1 , Zhijun Zhong  1 , Chao Gong  1 , Xuefeng Cao  1 , Yuan Song  1 , Wuyou Wang  1 , Xiangming Huang  1 , Xuehan Liu  1 , Yanchun Hu  1 , Hualin Fu  1 , Min He  1 , Ya Wang  1 , Yue Zhang  2 , Kongju Wu  2 , Guangneng Peng  1
Affiliations

Occurrence and Genetic Characteristics of Cryptosporidium hominis and Cryptosporidium andersoni in Horses from Southwestern China

Lei Deng et al. J Eukaryot Microbiol. 2017 Sep.

Abstract

A total of 333 fecal specimens from horses in southwestern China were genotyped based on analysis of the small subunit rRNA (SSU rRNA) gene. Cryptosporidium hominis and Cryptosporidium andersoni were identified in 2 and 4 stool specimens, respectively. The identification of C. hominis was confirmed by sequence analysis of the 70-kDa heat shock protein (HSP70) and oocyst wall protein (COWP) genes. Subtyping analysis of the 60-kDa glycoprotein (GP60) gene sequence of C. hominis revealed a new rare subtype Id, named IdA15; only three Id isolates have been reported in humans to date. Multilocus sequence typing (MLST) analysis indicated that the C. andersoni subtype was A6, A5, A2, and A1 at the four minisatellite loci (MS1, MS2, MS3, and MS16, respectively). This is the first report to identify the presence of C. andersoni and C. hominis in horses in southwestern China and the first to identify a rare zoonotic subtype Id of C. hominis in horses. These findings suggest that infected horses may act as potential reservoirs of Cryptosporidium to transmit infections to humans.

Keywords: GP60; multilocus sequence typing; transmission.

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Figures

Figure 1
Figure 1
Phylogenetic relationship of 60‐kDa glycoprotein (GP60) nucleotide sequences of the Cryptosporidium equine isolate in this study to multiple subtype families in C. hominis, as inferred by a neighbor‐joining analysis based on evolutionary distances calculated using the Kimura two‐parameter model. The tree was rooted with partial subtypes of C. fayeri. Bootstrap values were obtained using 1,000 pseudo‐replicates, with only values above 50% reported.
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References

    1. Burton, A. J. , Nydam, D. V. , Dearen, T. K. , Mitchell, K. , Bowman, D. D. & Xiao, L. 2010. The prevalence of Cryptosporidium, and identification of the Cryptosporidium horse genotype in foals in New York State. Vet. Parasitol., 174:139–144. - PubMed
    1. Cacciò, S. M. 2005. Molecular epidemiology of human cryptosporidiosis. Parassitologia, 47:185–192. - PubMed
    1. Current, W. L. , Reese, N. C. , Ernst, J. V. , Bailey, W. S. , Heyman, M. B. & Weinstein, W. M. 1983. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission. N. Engl. J. Med., 308:1252–1257. - PubMed
    1. Fayer, R. , Morgan, U. & Upton, S. J. 2000. Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol., 30:1305–1322. - PubMed
    1. Feng, Y. , Yang, W. , Ryan, U. , Zhang, L. , Kváč, M. , Koudela, B. , Modrý, D. , Li, N. , Fayer, R. & Xiao, L. 2011. Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni . J. Clin. Microbiol., 49:34–41. - PMC - PubMed

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