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. 2017 Mar;23(3):266-268.
doi: 10.1111/cns.12674. Epub 2017 Feb 6.

Cpne5 is Involved in Regulating Rodent Anxiety Level

Xue-Feng Ding  1   2 , He-Jun Wang  3 , Lu Qian  1 , Zeng-Yao Hu  4 , Shu-Fang Feng  1 , Yan Wu  2 , Hai-Hong Yang  1 , Hai-Tao Wu  1 , Wen-Hong Fan  5 , Ming Fan  2
Affiliations

Cpne5 is Involved in Regulating Rodent Anxiety Level

Xue-Feng Ding et al. CNS Neurosci Ther. 2017 Mar.
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Generation of Cpne5 knockout (KO) mouse model. A: The schematic diagram of strategy for preparing Cpne5 KO mice. Cpne5 will be inactivated by replacing the first exon of Cpne5 and its upstream regulation sequence with a PGK‐Neo‐cassette via homologous recombination. B: Ninety‐six ES clones were picked up, and five positive clones of them were characterized by PCR analysis. Targeting vector was used as positive control (PC), and 129/SvJ genomic DNA was used as negative control (NC). C: The positive clones were confirmed by Southern blot; a 9.7‐kb and a 6.7‐kb band will be observed in the KO‐positive clones. D: PCR analysis of chimeric offspring. Totally six heterozygotes were found in chimeric offsprings (519‐bp band will be observed in heterozygotes). E: Confirmation of Cpne5 homozygotes using immunofluorescence. Cpne5 cannot be observed in the adult hippocampus of homozygotes compared with wild‐type mice. Nucleus was stained with DAPI. Scale bars: 50 μm.
Figure 2
Figure 2
Behavioral characterization of wild‐type (n = 7) and Cpne5 knockout mice (n = 11). A: There is no significant of travel distance in open field task. B:There is no significant difference of time spent in the dark box of light–dark box task (LDB) was found between Cpne5 KO and WT mice. C: Cpne5 KO mice spent significantly more time in the open arm of elevated plus maze (EPM) compared with WT mice, P < 0.05, Percentage = time in open arms/(time in open arms + time in closed arms. D: Cpne5 KO mice spent significantly more time in the outer zone (P < 0.001) and less time in the center and middle zone of elevated platform (EP) compared with WT mice.
See this image and copyright information in PMC

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