Actin cytoskeleton and exocytosis in rat melanotrophs
- PMID: 28176104
- DOI: 10.1007/s004240000125
Actin cytoskeleton and exocytosis in rat melanotrophs
Abstract
We monitored secretory activity of single rat melanotrophs by the patch-clamp membrane capacitance measurements (Cm). Secretory activity was stimulated by cytosol dialysis with a patch-pipette solution containing 1μM [Ca2+]i. Actin cytoskeleton was disaggregated by pretreating cells with Clostridium spiroforme toxin, which specifically ADP-ribosylates cellular actin. The extent of cytoskeleton disaggregation was monitored by phalloidin immunostaining. The maximal rate of secretion increases two folds in toxin-treated cells in comparison to controls, whereas the extent of calcium-induced secretory response was similar to that obtained in the non-treated cells. The results show that the subcortical actin network attenuates the rate of secretory activity, which we interpret to reflect a barrier function of cytoskeleton for exocytosis.
Keywords: Key words actin cytoskeleton; endocytosis; exocytosis; melanotrophs; patch-clamp.
Similar articles
-
Actin cytoskeleton and exocytosis in rat melanotrophs.Pflugers Arch. 2000;439(3 Suppl):R148-9. Pflugers Arch. 2000. PMID: 10653173
-
Actin cytoskeleton depolymerization with clostridium spiroforme toxin enhances the secretory activity of rat melanotrophs.J Physiol. 1999 Dec 1;521 Pt 2(Pt 2):389-95. doi: 10.1111/j.1469-7793.1999.00389.x. J Physiol. 1999. PMID: 10581310 Free PMC article.
-
Distinct effect of actin cytoskeleton disassembly on exo- and endocytic events in a membrane patch of rat melanotrophs.J Physiol. 2002 Dec 15;545(3):879-86. doi: 10.1113/jphysiol.2002.028043. J Physiol. 2002. PMID: 12482893 Free PMC article.
-
Dynamic changes in chromaffin cell cytoskeleton as prelude to exocytosis.Mol Neurobiol. 1992 Winter;6(4):339-58. doi: 10.1007/BF02757940. Mol Neurobiol. 1992. PMID: 1337454 Review.
-
New insights into the role of the cortical cytoskeleton in exocytosis from neuroendocrine cells.Int Rev Cell Mol Biol. 2012;295:109-37. doi: 10.1016/B978-0-12-394306-4.00009-5. Int Rev Cell Mol Biol. 2012. PMID: 22449488 Review.
Cited by
-
Nucleotide dependent differences between the alpha-skeletal and alpha-cardiac actin isoforms.Biochem Biophys Res Commun. 2008 Apr 11;368(3):696-702. doi: 10.1016/j.bbrc.2008.01.158. Epub 2008 Feb 7. Biochem Biophys Res Commun. 2008. PMID: 18261974 Free PMC article.
-
Phospholipase Cη2 Activation Redirects Vesicle Trafficking by Regulating F-actin.J Biol Chem. 2015 Nov 27;290(48):29010-21. doi: 10.1074/jbc.M115.658328. Epub 2015 Oct 2. J Biol Chem. 2015. PMID: 26432644 Free PMC article.
-
The role of actin remodeling in the trafficking of intracellular vesicles, transporters, and channels: focusing on aquaporin-2.Pflugers Arch. 2008 Jul;456(4):737-45. doi: 10.1007/s00424-007-0404-2. Epub 2007 Dec 8. Pflugers Arch. 2008. PMID: 18066585 Review.
LinkOut - more resources
Full Text Sources
Miscellaneous
