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Review
. 2017 Jun;80(6):590-598.
doi: 10.1002/jemt.22834. Epub 2017 Feb 9.

Resolution and super-resolution

Affiliations
Review

Resolution and super-resolution

Colin J R Sheppard. Microsc Res Tech. 2017 Jun.

Abstract

Many papers have claimed the attainment of super-resolution, i.e. resolution beyond that achieved classically, by measurement of the profile of a feature in the image. We argue that measurement of the contrast of the image of a dark bar on a bright background does not give a measure of resolution, but of detection sensitivity. The width of a bar that gives an intensity at the center of the bar of 0.735 that in the bright region (the same ratio as in the Rayleigh resolution criterion) is λ/(13.9×numerical aperture) for the coherent case with central illumination. This figure, which compares with λ/(numerical aperture) for the Abbe resolution limit with central illumination, holds for the classical case, and so is no indication of super-resolution. Theoretical images for two points, two lines, arrays of lines, arrays of bars, and grating objects are compared. These results can be used a reference for experimental results, to determine if super-resolution has indeed been attained. The history of the development of the theory of microscope resolution is outlined.

Keywords: imaging theory; microscopy; resolution; super-resolution.

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