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Comment

. 2017 Feb 10;355(6325):582-584.

doi: 10.1126/science.aam5409.

Flipping nanoscopy on its head

Jie Xiao¹, Taekjip Ha^{1

2

3

4}

Affiliations

Affiliations

¹ Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA. xiao@jhmi.edu tjha@jhu.edu.
² Department of Biophysics, Johns Hopkins University, Baltimore, MD 21205, USA.
³ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
⁴ Howard Hughes Medical Institute, Baltimore, MD 21205, USA.

PMID: 28183938
PMCID: PMC8989063
DOI: 10.1126/science.aam5409

Comment

Flipping nanoscopy on its head

Jie Xiao et al. Science. 2017.

. 2017 Feb 10;355(6325):582-584.

doi: 10.1126/science.aam5409.

Authors

Jie Xiao¹, Taekjip Ha^{1

2

3

4}

Affiliations

¹ Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA. xiao@jhmi.edu tjha@jhu.edu.
² Department of Biophysics, Johns Hopkins University, Baltimore, MD 21205, USA.
³ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
⁴ Howard Hughes Medical Institute, Baltimore, MD 21205, USA.

PMID: 28183938
PMCID: PMC8989063
DOI: 10.1126/science.aam5409

No abstract available

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Figures

None — Seeing better in dimmer light
The MINFLUX method uses minimal excitation to resolve changes in molecular position within an excitation pattern. In common nanoscopy, a molecule’s position is determined near the maximum of the excitation intensity profile whereas in MINFLUX, it is determined near the minimum, requiring much fewer photons. The position of a molecule (red circle) is probed by watching it brighten and dim as the donut-shaped excitation profile is moved around it. Dark gray is brightest excitation.

See this image and copyright information in PMC

Comment on

Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes.
Balzarotti F, Eilers Y, Gwosch KC, Gynnå AH, Westphal V, Stefani FD, Elf J, Hell SW. Balzarotti F, et al. Science. 2017 Feb 10;355(6325):606-612. doi: 10.1126/science.aak9913. Epub 2016 Dec 22. Science. 2017. PMID: 28008086

References

1. Eggeling C, Willig KI, Sahl SJ, Hell SW, Q. Rev. Biophys 48, 178 (2015). - PubMed
1. Balzarotti F et al. Science 355, 606 (2017). - PubMed
1. Yildiz A et al. Science 300, 2061 (2003). - PubMed
1. Betzig E et al. Science 313, 1642 (2006). - PubMed
1. Rust MJ, Bates M, Zhuang X, Nat. Methods 3, 793 (2006). - PMC - PubMed

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