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. 2017:1550:11-18.
doi: 10.1007/978-1-4939-6747-6_2.

Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments

Jonathan Erde  1 , Rachel R Ogorzalek Loo  2 , Joseph A Loo  3   4
Affiliations

Improving Proteome Coverage and Sample Recovery with Enhanced FASP (eFASP) for Quantitative Proteomic Experiments

Jonathan Erde et al. Methods Mol Biol. 2017.

Abstract

Enhanced Filter Aided Sample Preparation (eFASP) incorporates plastics passivation and digestion-enhancing surfactants into the traditional FASP workflow to reduce sample loss and increase hydrophobic protein representation in qualitative and quantitative proteomics experiments. Resulting protein digests are free of contaminants and can be analyzed directly by LC-MS.

Keywords: Ammonium deoxycholate; Detergent; Enhanced filter aided sample preparation; Quantitative proteomics.

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References

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