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. 2017 Apr;78(4):336-341.
doi: 10.1016/j.humimm.2017.02.003. Epub 2017 Feb 9.

Relationship between Mean Fluorescence Intensity and C1q/C3d-fixing capacities of anti-HLA antibodies

Guillaume Claisse  1 , Lena Absi  2 , Fabrice Cognasse  3 , Eric Alamartine  4 , Christophe Mariat  5 , Nicolas Maillard  6
Affiliations

Relationship between Mean Fluorescence Intensity and C1q/C3d-fixing capacities of anti-HLA antibodies

Guillaume Claisse et al. Hum Immunol. 2017 Apr.

Abstract

Background: Complement-binding assays are proposed to better stratify the risk of antibody-mediated rejection associated-graft failure. Despite promising clinical results, some have suggested that the MFI of anti-HLA antibodies may influence these tests.

Methods: We investigated the impact of Abs MFI reduction, induced by plasmapheresis, on C1q- and C3d-binding assays. Sera provided from 7 sensitized kidney transplant patients were analyzed.

Results: Four hundreds and thirty-three SABs were analyzed. Before plasmapheresis, when compared to C1q- SABs, C1q+ SABs had a higher median MFI [17397 (IQR: 14851-18794) vs. 2745 (IQR: 1125-6476), p<0.01]. SABs that remained C1q+ after plasmapheresis had a higher median MFI. Regarding the C3d assay, results were strictly comparable. MFI value was a powerful predictor of both C1q and C3d positivity [AUC 0.97 (CI95% 0.95-0.99) and 0.96, (CI95% 0.93-0.98), respectively].

Conclusion: Our data suggest that both C1q- and C3d-binding assays are intimately linked to the MFI of anti-HLA Abs.

Keywords: C1q; C3d; LUMINEX; MFI; PROZONE.

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