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. 2017 Feb 15;12(2):e0171934.
doi: 10.1371/journal.pone.0171934. eCollection 2017.

Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta

Ran Zhang  1 , Shan Dong  1 , Wei-Wei Ma  1 , Xue-Ping Cai  1 , Zhi-Yin Le  1 , Rong Xiao  1 , Qi Zhou  2 , Huan-Ling Yu  1
Affiliations

Modulation of cholesterol transport by maternal hypercholesterolemia in human full-term placenta

Ran Zhang et al. PLoS One. .

Abstract

The significance of maternal cholesterol transporting to the fetus under normal as well as pathological circumstances is less understood. The objective of this study was to observe the effects of maternal hypercholesterolemia on placental cholesterol transportation. Human full-time placenta, maternal and venous cord blood were sampled at delivery from the pregnant women with serum total cholesterol (TC) concentrations at third trimester higher than 7.25 mM (n = 19) and the pregnant women with normal TC concentrations (n = 19). Serum lipids and expression of genes related to cholesterol transportation were measured by western blot or real-time PCR. The results indicated that serum TC, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) levels were significantly increased, in pregnancies, but decreased in cord blood in hypercholesterolemic group compared to the matched control group. All the subjects were no-drinking, non-smoker, and gestational disease free. The mRNA expression of lipoprotein receptors, including LDLR and VLDLR were significantly increased, while the protein expression of PCSK9 was significantly increased in hypercholesterolemic placenta. In conclusion, maternal hypercholesterolemia might decrease the transportation of cholesterol from mother to fetus because of the high levels of PCSK9 protein expression.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Influence of maternal hypercholesterolemia on cord blood lipid levels.
* means comparing with control group P<0.05.
Fig 2
Fig 2. Correlation analysis between maternal serum cholesterol (third trimester) concentration and artery cord blood cholesterol concentration.
Results are expressed as Pearson’s correlation and the curve represent Pearson’s linear correlation.
Fig 3
Fig 3. Expressions of genes related to cholesterol transporting in placenta.
Real-time PCR analysis of ABCG1, LXRβ, LXRα, SRBI, PPARγ, VLDLR, ABCA1 and LDLR genes expression from placenta of control and hypercholesterolemia groups. Results are the means ± SE (n = 19).
Fig 4
Fig 4. Pcsk9 protein expression (western blot, A) and mRNA expression (real-time PCR, B) in placenta from control and hypercholesterolemic pregnancy.
Results are the means ± SEM (n = 17). *p < 0.01 compared to control group.
See this image and copyright information in PMC

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