Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography
- PMID: 2820130
- DOI: 10.1016/0042-6822(87)90063-8
Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography
Abstract
Using high resolution electron microscopic autoradiography and immunocytochemistry with monoclonal antibodies against poliovirus proteins of the P2 genomic region, the location of these proteins in respect to the virus-induced vesicle formation and the viral RNA synthesis was followed during the viral replication cycle. It was found that P2 proteins become rER associated soon after their synthesis. At the site of protein and rER interaction, electron-dense patches appear. Simultaneously, membrane protrusions grow and form vesicles which finally budd off, carrying the patches on their outer surface. As shown by autoradiography, these patches are the site of viral RNA replication and, therefore, they represent the poliovirus replication complex. The vesicles with the replication complex, including replicating and replicated viral RNA, move away from the rER to form a continuously growing vesiculated area in the center of the infected cell, where virus maturation takes place. A likely function of the 2C protein is to attach the replication complex, or some of its components, to the vesicular membranes.
Similar articles
-
Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region.J Virol. 1990 Mar;64(3):1156-63. doi: 10.1128/JVI.64.3.1156-1163.1990. J Virol. 1990. PMID: 2154600 Free PMC article.
-
Formation of the poliovirus replication complex requires coupled viral translation, vesicle production, and viral RNA synthesis.J Virol. 2000 Jul;74(14):6570-80. doi: 10.1128/jvi.74.14.6570-6580.2000. J Virol. 2000. PMID: 10864671 Free PMC article.
-
Intracellular localization of poliovirus RNA by in situ hybridization at the ultrastructural level using single-stranded riboprobes.Virology. 1992 Dec;191(2):687-97. doi: 10.1016/0042-6822(92)90244-j. Virology. 1992. PMID: 1333118
-
Involvement of cellular membrane traffic proteins in poliovirus replication.Cell Cycle. 2007 Jan 1;6(1):36-8. doi: 10.4161/cc.6.1.3683. Epub 2007 Jan 11. Cell Cycle. 2007. PMID: 17245115 Review.
-
The Role of Electron Microscopy in Studying the Continuum of Changes in Membranous Structures during Poliovirus Infection.Viruses. 2015 Oct 12;7(10):5305-18. doi: 10.3390/v7102874. Viruses. 2015. PMID: 26473912 Free PMC article. Review.
Cited by
-
Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis.J Virol. 2012 Sep;86(18):9964-75. doi: 10.1128/JVI.00914-12. Epub 2012 Jul 3. J Virol. 2012. PMID: 22761387 Free PMC article.
-
Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment.J Virol. 2013 Oct;87(19):10612-27. doi: 10.1128/JVI.01370-13. Epub 2013 Jul 24. J Virol. 2013. PMID: 23885072 Free PMC article.
-
Evidence for the role of the P2 protein of human rhinovirus in its host range change.J Virol. 1989 May;63(5):2396-9. doi: 10.1128/JVI.63.5.2396-2399.1989. J Virol. 1989. PMID: 2539529 Free PMC article.
-
Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture.J Virol. 1992 Feb;66(2):650-4. doi: 10.1128/JVI.66.2.650-654.1992. J Virol. 1992. PMID: 1309907 Free PMC article.
-
cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication.J Virol. 1992 Oct;66(10):6045-57. doi: 10.1128/JVI.66.10.6045-6057.1992. J Virol. 1992. PMID: 1326655 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials