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Comment
. 2017 Feb 16:6:e25001.
doi: 10.7554/eLife.25001.

Micromanaging checkpoint proteins

Andrea Ciliberto  1 , Silke Hauf  2   3
Affiliations
Comment

Micromanaging checkpoint proteins

Andrea Ciliberto et al. Elife. .

Abstract

The kinase Mps1, long known to be the 'boss' in mitotic checkpoint signaling, phosphorylates multiple proteins in the checkpoint signaling cascade.

Keywords: E. coli; S. cerevisiae; S. pombe; biochemistry; cell biology; cell cycle; human; kinetochore; protein kinase; spindle checkpoint; xenopus.

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Conflict of interest statement

The authors declare that no competing interests exist.

Figures

Figure 1.
Figure 1.. The kinase Mps1 and its role in mitotic checkpoint signaling.
(A) Mps1 phosphorylates (P) three different proteins to promote the assembly of the mitotic checkpoint complex. It phosphorylates the kinetochore protein KNL1 to recruit the checkpoint protein complex Bub1-Bub3 to KNL1 (1). It phosphorylates Bub1, which allows this protein to interact with another checkpoint protein, Mad1 (2). It also phosphorylates Mad1, which promotes the binding of Mad2 to the regulatory protein Cdc20 (3). Ji et al. propose that phosphorylated Mad1 binds to Cdc20, thereby positioning the latter for capture by Mad2. (B) The checkpoint (represented by the STOP sign) is only active when Mps1 has phosphorylated all three proteins, KNL1, Bub1, and Mad1. (C) Checkpoint activity (y-axis) plotted as a function of Mps1 kinase activity (x-axis) for the phosphorylation of one (P), two (PP) or all three sites (PPP).
See this image and copyright information in PMC

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