doi: 10.1016/0014-5793(87)80197-7.
Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases
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- PMID: 2820801
- DOI: 10.1016/0014-5793(87)80197-7
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Cleavage and sequence recognition of 2,6-diaminopurine-containing DNA by site-specific endonucleases
FEBS Lett.
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- FEBS Lett 1987 Nov 2;223(2):424
Abstract
The susceptibility of 2,6-diaminopurine (DAP)-containing bacteriophage DNA to several restriction and other endonucleases was examined. With the only exception of TaqI, these enzymes did not accept the modified base as a substitute for adenine. The phage DNA was extensively fragmented by the restriction endonucleases which recognize only G and C-containing sites. 5'-terminal analysis of the MspI and SmaI fragments revealed that d(DAP-T) basepairs can be mistaken by some enzymes for d(G-C) pairs.
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