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. 1987 Aug;52(3):273-8.
doi: 10.1016/0303-7207(87)90055-4.

Dual activation by thyrotropin of the phospholipase C and cyclic AMP cascades in human thyroid

E Laurent  1 , J Mockel, J Van Sande, I Graff, J E Dumont
Affiliations

Dual activation by thyrotropin of the phospholipase C and cyclic AMP cascades in human thyroid

E Laurent et al. Mol Cell Endocrinol. 1987 Aug.

Abstract

In human thyroid slices prelabeled with myo-[2-3H]inositol, thyrotropin (TSH, 3-30 mU/ml) stimulated IP3, IP2 and IP1 generation over a prolonged time course. The cAMP response was much more sensitive to TSH, peaking between 1 and 5 mU/ml. Forskolin (10(-5) M) and isoproterenol had no effect on basal IP levels, while carbamylcholine (10(-5) M, 10(-4) M) also increased IP accumulation. These data suggest that in the human thyroid, TSH activates a phospholipase C generating IP3 and diacylglycerol independently of the well-known adenylate cyclase stimulation. They validate in the human model a dual mode of action of the hormone previously proposed on the basis of indirect observations.

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