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. 2016 Dec;10(12):ZC23-ZC26.
doi: 10.7860/JCDR/2016/20262.9044. Epub 2016 Dec 1.

Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma - A Histochemical Study

Wasim Raja Mumtaz  1 , Veda Hegde  2 , Nikhil Yadav  1
Affiliations

Micrometastasis Detection using Special Stains in Nodal Tissues of Oral Squamous Cell Carcinoma - A Histochemical Study

Wasim Raja Mumtaz et al. J Clin Diagn Res. 2016 Dec.

Abstract

Introduction: Micrometastasis to the lymph node is an important prognostic factor in survival and recurrence and even in making critical decisions regarding postoperative radiation treatment. Methods like Immunohistochemistry (IHC), serial sectioning and Polymerase Chain Reaction (PCR) are very sensitive; however, they are expensive and time consuming. Hence, there is a need for the detection of micrometastasis by method which is easily feasible and inexpensive.

Aim: This study was undertaken to identify if special stains (Modified Papanicolaou stain and Ayoub Shklar stain) are valuable in detecting micrometastasis which are routinely missed in Haematoxylin and Eosin stain and also to compare their efficacy over Haematoxylin and Eosin stain in detection of metastatic tumour cells in non-metastatic lymph nodes.

Materials and methods: This study constituted a total of 300 histopathologically proven non metastatic lymph node sections. Other than Haematoxylin and Eosin stain, Modified Papanicalaou and Ayoub Shklar stain were used for identification of micrometastatic deposit.

Results: Papanicolaou stain was the only stain useful in detecting micrometastasis which accounted to 7% of non metastatic lymph node sections used in our study.

Conclusion: Papanicolaou stain has definitely proved valuable in detecting micrometastasis over routine Haematoxylin and Eosin and Ayoub Shklar stain with an added advantage of being economical, easily available and technique insensitive.

Keywords: Haematoxylin and Eosin stain; Lymph nodes; Metastasis; Papanicoloaou stain.

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Figures

[Table/Fig-1]:
[Table/Fig-1]:
Photomicrograph reveals micrometastatic deposits in subcapsular area stained by Papanicolaou stain (x40).
[Table/Fig-2]:
[Table/Fig-2]:
Photomicrograph reveals micrometastatic deposits in lymph node stained by Papanicolaou stain (x100).
[Table/Fig-4]:
[Table/Fig-4]:
Photomicrograph of the lymph node sections stained by Ayoub Shklar stain (x40).
[Table/Fig-5]:
[Table/Fig-5]:
Photomicrograph of the lymph node sections stained by Haematoxylin and Eosin stain (x40).
[Table/Fig-6]:
[Table/Fig-6]:
Comparison of the efficacy of all the three stains in detecting micro-metastasis in lymph node sections.
See this image and copyright information in PMC

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