Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp
- PMID: 2820946
- PMCID: PMC213867
- DOI: 10.1128/jb.169.10.4848-4851.1987
Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp
Abstract
A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl2 and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl2. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl2 or to PMA.
Similar articles
-
Cloning and expression of the mercury resistance genes of marine Pseudomonas sp. strain MR1 plasmid pMR1 in Escherichia coli.Res Microbiol. 1994 Feb;145(2):121-7. doi: 10.1016/0923-2508(94)90005-1. Res Microbiol. 1994. PMID: 8090992
-
Nucleotide sequence of a chromosomal mercury resistance determinant from a Bacillus sp. with broad-spectrum mercury resistance.J Bacteriol. 1989 Jan;171(1):83-92. doi: 10.1128/jb.171.1.83-92.1989. J Bacteriol. 1989. PMID: 2536669 Free PMC article.
-
Screening of mercury-resistant and indole-3-acetic acid producing bacterial-consortium for growth promotion of Cicer arietinum L.J Basic Microbiol. 2017 Mar;57(3):204-217. doi: 10.1002/jobm.201600352. Epub 2016 Dec 2. J Basic Microbiol. 2017. PMID: 27911010
-
Cloning and DNA sequence of the mercuric- and organomercurial-resistance determinants of plasmid pDU1358.Proc Natl Acad Sci U S A. 1987 May;84(10):3112-6. doi: 10.1073/pnas.84.10.3112. Proc Natl Acad Sci U S A. 1987. PMID: 3033633 Free PMC article.
-
Molecular cloning and analysis of Staphylococcus aureus chromosomal aminoglycoside resistance genes.Plasmid. 1986 Mar;15(2):104-18. doi: 10.1016/0147-619x(86)90047-8. Plasmid. 1986. PMID: 3010352 Review.
Cited by
-
Five hundred years of mercury exposure and adaptation.J Biomed Biotechnol. 2012;2012:472858. doi: 10.1155/2012/472858. Epub 2012 Jul 15. J Biomed Biotechnol. 2012. PMID: 22910643 Free PMC article.
-
Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants.World J Microbiol Biotechnol. 1992 Jan;8(1):30-8. doi: 10.1007/BF01200680. World J Microbiol Biotechnol. 1992. PMID: 24425330
-
Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates.Appl Environ Microbiol. 1997 Mar;63(3):1066-76. doi: 10.1128/aem.63.3.1066-1076.1997. Appl Environ Microbiol. 1997. PMID: 9055422 Free PMC article.
-
Mercury resistance in Bacillus cereus RC607: transcriptional organization and two new open reading frames.J Bacteriol. 1999 Nov;181(22):7080-6. doi: 10.1128/JB.181.22.7080-7086.1999. J Bacteriol. 1999. PMID: 10559175 Free PMC article.
-
Cell-free mercury volatilization activity from three marine caulobacter strains.Appl Environ Microbiol. 1989 Feb;55(2):523-5. doi: 10.1128/aem.55.2.523-525.1989. Appl Environ Microbiol. 1989. PMID: 2655538 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources