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. 1987 Oct;169(10):4865-8.
doi: 10.1128/jb.169.10.4865-4868.1987.

Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34

D Nies  1 , M Mergeay, B Friedrich, H G Schlegel
Affiliations

Cloning of plasmid genes encoding resistance to cadmium, zinc, and cobalt in Alcaligenes eutrophus CH34

D Nies et al. J Bacteriol. 1987 Oct.

Abstract

A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.

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References

    1. J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. J Bacteriol. 1978 Jul;135(1):227-38 - PubMed
    1. Arch Int Physiol Biochim. 1978 May;86(2):440-2 - PubMed

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