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. 1987 Mar;133(3):683-90.
doi: 10.1099/00221287-133-3-683.

Isolation and characterization of Pseudomonas putida R-prime plasmids

R Bray  1 , D Strom, J Barton, H F Dean, A F Morgan
Affiliations

Isolation and characterization of Pseudomonas putida R-prime plasmids

R Bray et al. J Gen Microbiol. 1987 Mar.

Abstract

A number of enhanced chromosome mobilizing (ECM) plasmids derived from the wide host range plasmid R68 have been used to construct R-prime plasmids carrying a maximum of two map minutes of the Pseudomonas putida PPN chromosome, using Pseudomonas aeruginosa PAO as the recipient. For one ECM plasmid, pMO61, the ability to form R-primes did not correlate with the ability to mobilize chromosomes in intrastrain crosses, suggesting that different mechanisms are involved. Physical analysis of one R-prime showed that 3.5 kb of chromosomal DNA had been inserted between the tandem IS21 sequences carried by the parent ECM plasmid.

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