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. 2017 Apr;13(4):363-365.
doi: 10.1038/nchembio.2314. Epub 2017 Feb 20.

Engineering fatty acid synthases for directed polyketide production

Jan Gajewski  1 , Floris Buelens  2 , Sascha Serdjukow  3 , Melanie Janßen  1 , Niña Cortina  1 , Helmut Grubmüller  2 , Martin Grininger  1   3
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Engineering fatty acid synthases for directed polyketide production

Jan Gajewski et al. Nat Chem Biol. 2017 Apr.

Abstract

In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.

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