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. 1987 Jul;148(2):107-14.
doi: 10.1007/BF00425357.

Screening for plasmids in the genus Clostridium

C K Lee  1 , P Dürre, H Hippe, G Gottschalk
Affiliations

Screening for plasmids in the genus Clostridium

C K Lee et al. Arch Microbiol. 1987 Jul.

Abstract

A plasmid screening was performed on 150 strains out of 75 clostridial species using a modification of the alkaline-lysis procedure. In 26 strains representing 21 species one or more plasmid bands were detected ranging in size from 3 to more than 100 kilobase pairs. Clostridium aceticum proved to contain a single small plasmid (pCA1) of 5.4 kbp as revealed by restriction analysis and electron microscopy. A physical map of pCA1 has been constructed. Spontaneous mutants of C. aceticum defective in autotrophic growth have been isolated. No direct correlation between plasmid content and autotrophy could be found.

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