TLR4 rs41426344 increases susceptibility of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population

Abstract

Background: The aim of the study was to determine whether polymorphisms in toll-like receptor 4 (TLR4) confer susceptibility to rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) in a central south Chinese Han population.

Methods: Genotyping for six well studied polymorphisms (rs4986790, rs4986791, rs10759932, rs41426344, rs11536889 and rs7873784) in TLR4 gene were conducted in 1074 unrelated patients with RA and 1692 healthy control subjects, as well as in 217 unrelated patients with JIA and 378 healthy control subjects using direct sequencing technique. Comparisons between cases and controls in alleles, genotypes and haplotypes were carried out using Fisher's exact test.

Results: Significant genetic associations were detected between the 3'UTR rs41426344C and RA (p < 0.001, p _adj < 0.001, OR = 2.24) and JIA (p < 0.001, p _adj < 0.001, OR = 2.05). In addition, rs4986790G was found to be significantly associated with the susceptibility for RA (p = 0.005, p _adj = 0.03, OR = 3.43), but not for JIA (p = 0.06, p _adj = 0.36, OR = 2.65). Furthermore, significant increasing in the distributions of haplotypes H4 and H10 in RA (H4: p = 0.001, OR = 1.13; H10: p = 0.001, OR = 1.15) and JIA (H4: p = 0.04, OR = 2.06; H10: p = 0.02, OR = 2.47) were also found. Moreover, the frequency of rs41426344C significantly increased in RF-positive and anti-CCP positive subjects both in RA (RF⁺: p <0.0001, OR = 2.33; anti-CCP⁺: p =0.008, OR = 2.79) and JIA (RF⁺: p =0.02, OR = 2.91; anti-CCP⁺: p = 0.02, OR = 2.78).

Conclusions: Our study suggested that rs41426344 and rs4986790 of TLR4 might contribute to RA, and rs41426344 might contribute to JIA pathogenesis in central south Chinese Han population.

Keywords: Chinese Han population; Genetic association; Juvenile idiopathic arthritis (JIA); Rheumatoid arthritis (RA); Toll like receptors 4 (TLR4).