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. 2016 Dec;9(Suppl1):S70-S74.

First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis

Ahmad Hosseini-Safa  1 , Mohammad Ali Mohag Hegh  2 , Nader Pestechian  2 , Maryam Ganji  3 , Rasoul Mohammadi  2 , Reza Mahmoudi Lamouki  4 , Mohammad Rostami-Nejad  5
Affiliations

First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis

Ahmad Hosseini-Safa et al. Gastroenterol Hepatol Bed Bench. 2016 Dec.

Abstract

Aim: The present study was aimed to evaluate E. granulosus genotypes isolated from goats using HRM analysis in Isfahan province.

Background: Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage of Echinococcus granulosus. This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are important for the development of control strategies.

Methods: From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting curve (HRM) method.

Results: the results of HRM analysis using the sequence of CO1 gene for 43 Echinococcus granulosus isolates from goats showed 31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively.

Conclusion: G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was reported for the first time in goat in Iran.

Keywords: Echinococcus granulosus; G2 genotype; HRM; Iran; goat.

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Figures

Figure 1
Figure 1
Molecular phylogenetic tree of 10 E. granulosus isolates of goat along with reference isolates based on CO1 gene sequence. The evolutionary history was inferred by using the Maximum Likelihood method based on the Kimura 2 parameter model (23). The tree with the highest log likelihood (-814.2241) is shown. Reference accession nos.: G1, M64661; G2, M64662; G3, M64663; G6, M84666
Figure 2
Figure 2
HRM based on (EVA Green-TM) curve analyses of E. granulosus identified by sequencing (A-C). (A) G1, (B) G3, and (C) G2 genotype
Figure 3
Figure 3
Tm of the analyzed hydatid cysts. (A) E. granulosus G1 identified by sequencing, (B) E. granulosus G3 identified by sequencing, and (C) E. granulosus G2 identified by sequencing.
See this image and copyright information in PMC

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