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. 2017 Feb 7:7:30.
doi: 10.3389/fcimb.2017.00030. eCollection 2017.

The Escherichia coli Serogroup O1 and O2 Lipopolysaccharides Are Encoded by Multiple O-antigen Gene Clusters

Sabine Delannoy  1 , Lothar Beutin  2 , Patricia Mariani-Kurkdjian  3 , Aubin Fleiss  1 , Stéphane Bonacorsi  3 , Patrick Fach  1
Affiliations

The Escherichia coli Serogroup O1 and O2 Lipopolysaccharides Are Encoded by Multiple O-antigen Gene Clusters

Sabine Delannoy et al. Front Cell Infect Microbiol. .

Abstract

Escherichia coli strains belonging to serogroups O1 and O2 are frequently associated with human infections, especially extra-intestinal infections such as bloodstream infections or urinary tract infections. These strains can be associated with a large array of flagellar antigens. Because of their frequency and clinical importance, a reliable detection of E. coli O1 and O2 strains and also the frequently associated K1 capsule is important for diagnosis and source attribution of E. coli infections in humans and animals. By sequencing the O-antigen clusters of various O1 and O2 strains we showed that the serogroups O1 and O2 are encoded by different sets of O-antigen encoding genes and identified potentially new O-groups. We developed qPCR-assays to detect the various O1 and O2 variants and the K1-encoding gene. These qPCR assays proved to be 100% sensitive and 100% specific and could be valuable tools for the investigations of zoonotic and food-borne infection of humans with O1 and O2 extra-intestinal (ExPEC) or Shiga toxin-producing E. coli (STEC) strains.

Keywords: E. coli; ExPEC; O-AGC; O-antigen gene cluster; STEC; Shiga toxin-producing E. coli; extraintestinal E. coli; serotyping.

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Figures

Figure 1
Figure 1
The O-Antigen gene clusters of O1, O2 and O50 strains and variants thereof. The accession numbers of sequences obtained from public databases are indicated. The open arrows represent the location and orientation of the putative genes identified.
Figure 2
Figure 2
Phylogenetic analysis of the (A) wzx and (B) wzy nucleotide sequences of O1 strains. Phylogenetic trees were constructed using the CLC sequence viewer using the UPGMA algorithm. Reliability of the nodes is supported by bootstrap values > 70% (100 replicates).
Figure 3
Figure 3
Phylogenetic analysis of the (A) wzx and (B) wzy nucleotide sequences of O2/O50 strains. Phylogenetic trees were constructed using the CLC sequence viewer using the UPGMA algorithm. Reliability of the nodes is supported by bootstrap values of 100% (100 replicates).
See this image and copyright information in PMC

References

    1. Achtman M., Heuzenroeder M., Kusecek B., Ochman H., Caugant D., Selander R. K., et al. . (1986). Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infect. Immun. 51, 268–276. - PMC - PubMed
    1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., et al. . (1983). Six widespread bacterial clones among Escherichia coli K1 isolates. Infect. Immun. 39, 315–335. - PMC - PubMed
    1. Allison G. E., Verma N. K. (2000). Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri. Trends Microbiol. 8, 17–23. 10.1016/S0966-842X(99)01646-7 - DOI - PubMed
    1. Alonso M. Z., Sanz M. E., Irino K., Krüger A., Lucchesi P. M., Padola N. L. (2016). Isolation of atypical enteropathogenic Escherichia coli from chicken and chicken-derived products. Br. Poult. Sci. 57, 161–164. 10.1080/00071668.2015.1135502 - DOI - PubMed
    1. Bettelheim K. A., Beutin L., Gleier K., Pearce J. L., Luke R. K., Zimmermann S. (2003). Serotypes of Escherichia coli isolated from healthy infants in Berlin, Germany and Melbourne, Australia. Comp. Immunol. Microbiol. Infect. Dis. 26, 55–63. 10.1016/S0147-9571(02)00015-2 - DOI - PubMed

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