Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987 Oct;25(10):1822-9.
doi: 10.1128/jcm.25.10.1822-1829.1987.

Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization

J R Ticehurst  1 , S M Feinstone, T Chestnut, N C Tassopoulos, H Popper, R H Purcell
Affiliations
Comparative Study

Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization

J R Ticehurst et al. J Clin Microbiol. 1987 Oct.

Abstract

Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA.

PubMed Disclaimer

References

    1. J Virol. 1986 Oct;60(1):124-30 - PubMed
    1. J Infect Dis. 1986 Aug;154(2):231-7 - PubMed
    1. J Virol. 1987 Jan;61(1):50-9 - PubMed
    1. Ann Intern Med. 1987 Feb;106(2):221-6 - PubMed
    1. JAMA. 1970 Nov 9;214(6):1041-6 - PubMed

Publication types