Inferior olivary nuclear complex of the rat: morphology and comments on the principles of organization within the olivocerebellar system

Abstract

This study was undertaken (1) to provide a description of the normal morphology and anatomical interrelationships within the inferior olivary cell groups and (2) to determine the topographical organization of projections from this nuclear complex to the cerebellum. Conventional histological methods and the technique of retrograde transport of horseradish peroxidase (HRP) and WGA-HRP in conjunction with the sensitive chromogen tetramethyl benzidine were used in this series of experiments. In common with that of other animals, the inferior olivary nucleus of the rat consists of three subdivisions: the medial accessory olive (MAO), dorsal accessory olive (DAO), and principal olive (PO). The MAO is made of several subnuclei including a, b, c, and nucleus beta. On the basis of their common connections, the smaller subnuclei, dorsal cap, ventrolateral outgrowth, and dorsomedial cell column can be considered as parts of the MAO. The DAO is made of two subdivisions or lamellae--the dorsal and ventral folds--joined together laterally to form a twisted V-shaped structure. The principal olive consists of dorsal and ventral lamellae. A point-to-point projection was determined for four areas of the cerebellum: the anterior lobe, the posterior vermis, the intermediate and the lateral cerebellum. Analysis of the details of the projection system revealed that distinct groups of cells, here referred to as lamellae, project to sagittal zones of the cerebellum. (1) The medial accessory olive appears to be composed of three lamellae: horizontal, vertical, and rostral. The horizontal lamella (elsewhere denoted as groups a and b) projects to a sagittal zone in the vermal anterior lobe. The vertical lamella (groups c, beta, dorsal cap, ventrolateral outgrowth, and dorsomedial cell column) projects to a sagittal zone in the posterior vermis and the flocculus, and the rostral lamella projects to the lateral cerebellum. (2) The dorsal accessory olive is composed of two distinct lamellae which we have previously denoted as the dorsal and ventral folds. The dorsal fold projects to the vermal anterior lobe and receives afferents from the spinal cord, whereas the ventral fold projects to a sagittal zone in the intermediate cerebellum and receives afferents primarily from dorsal column nuclei. (3) The principal olive contains the two familiar lamellae: dorsal and ventral lamellae, each of which projects to a specific sagittal strip in the lateral cerebellum.(ABSTRACT TRUNCATED AT 400 WORDS)