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. 1987 Nov;61(11):3645-7.
doi: 10.1128/JVI.61.11.3645-3647.1987.

Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein

R Ostermayr  1 , K von der Helm, V Gauss-Müller, E L Winnacker, F Deinhardt
Affiliations

Expression of hepatitis A virus cDNA in Escherichia coli: antigenic VP1 recombinant protein

R Ostermayr et al. J Virol. 1987 Nov.

Abstract

The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with rabbit anti-HAV serum, suggesting that it possesses HAV antigenicity.

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