Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Nov;31(6):e22132.
doi: 10.1002/jcla.22132. Epub 2017 Feb 23.

A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin and β2 -microglobulin

Zhi Liu  1 , Jing Huang  1 , Rui-Ming Ou  1 , Meng-Dong Yao  1 , Yan-Lin She  2 , Rui Chen  2 , Cheng Li  2 , Li Xu  3 , Aikebaier Abudureyimu  3 , Qing Zhang  1 , Shuang Liu  1
Affiliations

A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin and β2 -microglobulin

Zhi Liu et al. J Clin Lab Anal. 2017 Nov.

Abstract

Background: Lymphocytic leukemia is a kind of primary malignant tumor of hematopoietic tissue. The aim was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of ferritin (FER) and β2 -microglobulin (β2 -MG) for the early screening and follow-up surveillance of lymphocytic leukemia.

Methods: The sandwich immunoassay was used to detect the concentration of FER, and the competitive immunoassay was used to detect the concentration of β2 -MG in serum. FER in serum was captured by anti- FER antibody immobilized on microtiter wells, and then banded together with another anti- FER labeled with europium(III) Eu3+ chelate, followed by fluorescence measurement using time-resolved fluorometry (TRF). Sm3+ labeled β2 -MG and β2 -MG samples were added to compete with a certain amount of anti-β2 -MG antibody, followed by fluorescence measurement using TRF. The performance of this dual-label TRFIA was evaluated using the clinical blood and compared with the commercial assays.

Results: The linear correlation coefficient (R2 ) of the FER and β2 -MG standard curves were 0.9914 and 0.9927, respectively. The sensitivity for FER detection was 8 ng/mL (dynamic range 0-1000 ng/mL), the average recovery was 100.51%; The sensitivity for β2 -MG detection was 1 ng/mL (dynamic range 0-1000 ng/mL), the average recovery was 101.02%. High correlation coefficients (R2 ) were obtained between the commercial assays (R2 =.9966 for FER, and R2 =.9897 for β2 -MG).

Conclusion: The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical sample analysis. It is an effective detection method for the early screening and follow-up surveillance of the acute and chronic lymphocytic leukemia.

Keywords: dual-label time-resolved fluorescence immunoassay; ferritin; lymphocytic leukemia; β2-microglobulin.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The standard curves of FER (A) and β2‐MG (B)
Figure 2
Figure 2
Correlation of FER (A) and β2‐MG (B) results between the dual‐labeled TRFIA and the commercial kits
See this image and copyright information in PMC

References

    1. Pui C, Robison LL, Look AT. Acute lymphoblastic leukaemia. Lancet. 2008;371:1030–1043. - PubMed
    1. Sikaria S, Aldoss I, Akhtari M. Monoclonal antibodies and immune therapies for adult precursor B‐acute lymphoblastic leukemia. Immunol Lett. 2016;172:113–123. - PubMed
    1. Rowe JM, Buck G, Burnett AK, et al. Induction therapy for adults with acute lymphoblastic leukemia: results of more than 1500 patients from the international ALL trial: MRC UKALL XII/ECOG E2993. Blood. 2005;106:3760–3767. - PubMed
    1. Fielding AK, Richards SM, Chopra R, et al. Outcome of 609 adults after relapse of acute lymphoblastic leukemia (ALL); an MRC UKALL12/ECOG 2993 study. Blood. 2005;109:944–950. - PubMed
    1. Ghia P, Ferreri AJM, Caligaris‐Cappio F. Chronic lymphocytic leukemia. Crit Rev Oncol Hematol. 2007;64:234–246. - PubMed

LinkOut - more resources