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. 2017 Feb 23;17(1):20.
doi: 10.1186/s12896-017-0340-y.

Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Jun Xia  1 , Jiaxing Xu  2 , Xiaoyan Liu  2 , Jiming Xu  2 , Xingfeng Wang  2 , Xiangqian Li  3
Affiliations

Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Jun Xia et al. BMC Biotechnol. .

Abstract

Background: poly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-scale production. To overcome this problem, PMA production from Jerusalem artichoke tubers was successfully performed. Additionally, a valuable exopolysaccharide, pullulan, was co-produced with PMA by Aureobasidum pullulans HA-4D.

Results: The Jerusalem artichoke medium for PMA and pullulan co-production contained only 100 g/L hydrolysate sugar, 30 g/L CaCO3 and 1 g/L NaNO3. Compared with the glucose medium, the Jerusalem artichoke medium resulted in a higher PMA concentration (114.4 g/L) and a lower pullulan concentration (14.3 g/L) in a 5 L bioreactor. Meanwhile, the activity of pyruvate carboxylase and malate dehydrogenas was significantly increased, while the activity of α-phosphoglucose mutase, UDP-glucose pyrophosphorylase and glucosyltransferase was not affected. To assay the economic-feasibility, large-scale production in a 1 t fermentor was performed, yielding 117.5 g/L PMA and 15.2 g/L pullulan.

Conclusions: In this study, an economical co-production system for PMA and pullulan from Jerusalem artichoke was developed. The medium for PMA and pullulan co-production was significantly simplified when Jerusalem artichoke tubers were used. With the simplified medium, PMA production was obviously stimulated, which would be associated with the improved activity of pyruvate carboxylase and malate dehydrogenas.

Keywords: Co-production; Enzyme activity; Jerusalem artichoke; Poly(malic acid); Pullulan.

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Figures

Fig. 1
Fig. 1
Effect of initial sugar concentration derived from Jerusalem artichoke tuber hydrolysate on PMA and pullulan co-production in shake-flasks
Fig. 2
Fig. 2
Precipitation of exopolysaccharide with the addition of ethanol (a) and HPLC analysis of the supernatant after the removal of exopolysaccharide (b)
Fig. 3
Fig. 3
PMA and pullulan co-production with JAT medium in fed-batch fermentation by A.pulluans HA-4D. a 5 L fermentor; (b) 1 t fermentor
Fig. 4
Fig. 4
The proposed pathway of PMA and pullulan metabolism in A.pullulans
See this image and copyright information in PMC

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