Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Nov;84(21):7388-92.
doi: 10.1073/pnas.84.21.7388.

Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5

T Yokota  1 , R L Coffman, H Hagiwara, D M Rennick, Y Takebe, K Yokota, L Gemmell, B Shrader, G Yang, P Meyerson, et al.
Affiliations

Isolation and characterization of lymphokine cDNA clones encoding mouse and human IgA-enhancing factor and eosinophil colony-stimulating factor activities: relationship to interleukin 5

T Yokota et al. Proc Natl Acad Sci U S A. 1987 Nov.

Abstract

Conditioned medium from the Con A-treated mouse helper T-cell clone Ly1+2-/9 contains activities that enhance the production of IgA by mouse B cells and induce human cord blood cells to form eosinophil colonies. We have isolated a cDNA sequence that expresses IgA-enhancing factor and eosinophil colony-stimulating factor activities from a cDNA library prepared from activated Ly1+2-/9 cells. Based on homology with the mouse cDNA sequence, a human cDNA sequence coding for an interleukin with IgA-enhancing factor and eosinophil colony-stimulating factor activities was isolated from a cDNA library prepared from a human T-cell clone stimulated with anti-T3 antibody and phorbol 12-myristate 13-acetate. DNA sequence analyses revealed that mouse and human cDNA clones encode proteins of 133 and 134 amino acids, respectively, that are identical to cDNA clones encoding the T-cell replacing factor I and B-cell growth factor II activities. These results establish that a single cDNA clone encodes a protein that acts as a growth and differentiation factor for both B cells and eosinophils.

PubMed Disclaimer

References

    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Nucleic Acids Res. 1986 Nov 25;14(22):9149-58 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1157-61 - PubMed
    1. Mol Cell Biol. 1983 Feb;3(2):280-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1070-4 - PubMed