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. 1987 Jul;164(1):53-9.
doi: 10.1016/0003-2697(87)90366-6.

Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis

J J Kupiec  1 , M L Giron, D Vilette, J M Jeltsch, R Emanoil-Ravier
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Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis

J J Kupiec et al. Anal Biochem. 1987 Jul.

Abstract

A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.

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