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. 1987 Sep;53(9):2256-9.
doi: 10.1128/aem.53.9.2256-2259.1987.

Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization

A R Datta  1 , B A Wentz, W E Hill
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Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization

A R Datta et al. Appl Environ Microbiol. 1987 Sep.

Abstract

A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.

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References

    1. Am J Vet Res. 1974 Feb;35(2):289-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 - PubMed
    1. J Clin Microbiol. 1977 Jun;5(6):559-63 - PubMed
    1. Ann Microbiol (Paris). 1980 Jul-Aug;131B(1):47-57 - PubMed
    1. J Clin Microbiol. 1982 Mar;15(3):503-7 - PubMed

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