. 2017 Apr;116(4):1247-1256.

doi: 10.1007/s00436-017-5401-6. Epub 2017 Feb 25.

Preliminary genetic evidence of two different populations of Opisthorchis viverrini in Lao PDR

Opal Pitaksakulrat^{1

2

3}, Nadda Kiatsopit^{1

2}, Nonglak Laoprom^{1

2

4}, Bonnie L Webster^{5

6}, Joanne P Webster^{7

8}, Poppy H L Lamberton⁹, Thewarach Laha¹, Ross H Andrews^{1

2

7

10}, Trevor N Petney^{10

11}, David Blair¹², Elizabeth J Carlton¹³, Robert C Spear¹⁴, Paiboon Sithithaworn^{15

16}

Affiliations

¹ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
² Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
³ Faculty of Veterinary Science, Prince of Songkla University, Songkhla, 90110, Thailand.
⁴ Department of General Science, Faculty of Science and Engineering, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.
⁵ Department of Life Sciences, Parasites and Vectors Division, Natural History Museum, London, SW7 5BD, UK.
⁶ The London Centre for Neglected Tropical Disease Research (LCNTDR), Imperial College, Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK.
⁷ Department of Infectious Disease Epidemiology, Imperial College, Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK.
⁸ Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertfordshire, London, AL9 7TA, UK.
⁹ Institute of Biodiversity, Animal Health and Comparative Medicine, and Wellcome Centre for Molecular Parasitology, Graham Kerr Building, The University of Glasgow, Glasgow, G12 8QQ, UK.
¹⁰ Cholangiocarcinoma Screening and Care Program, (CASCAP) Khon Kaen University, Khon Kaen, 40002, Thailand.
¹¹ Institute of Zoology 1: Ecology and Parasitology, Karlsruhe Institute of Technology, KornblumenStrasse 13, Karlsruhe, Germany.
¹² Centre for Tropical Diversity and Climate change, James Cook University, Townsville, Australia.
¹³ Department of Environmental and Occupational Health, Colorado School of Public Health, 13001 E. 17th Place, B119, Aurora, CO, 8004510, USA.
¹⁴ Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA, USA.
¹⁵ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. paib_sit@kku.ac.th.
¹⁶ Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. paib_sit@kku.ac.th.

PMID: 28238124
PMCID: PMC5756626
DOI: 10.1007/s00436-017-5401-6

Preliminary genetic evidence of two different populations of Opisthorchis viverrini in Lao PDR

Opal Pitaksakulrat et al. Parasitol Res. 2017 Apr.

. 2017 Apr;116(4):1247-1256.

doi: 10.1007/s00436-017-5401-6. Epub 2017 Feb 25.

Authors

Affiliations

¹ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
² Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
³ Faculty of Veterinary Science, Prince of Songkla University, Songkhla, 90110, Thailand.
⁴ Department of General Science, Faculty of Science and Engineering, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, 47000, Thailand.
⁵ Department of Life Sciences, Parasites and Vectors Division, Natural History Museum, London, SW7 5BD, UK.
⁶ The London Centre for Neglected Tropical Disease Research (LCNTDR), Imperial College, Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK.
⁷ Department of Infectious Disease Epidemiology, Imperial College, Faculty of Medicine (St Mary's Campus), Norfolk Place, London, W2 1PG, UK.
⁸ Department of Pathology and Pathogen Biology, Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, Hertfordshire, London, AL9 7TA, UK.
⁹ Institute of Biodiversity, Animal Health and Comparative Medicine, and Wellcome Centre for Molecular Parasitology, Graham Kerr Building, The University of Glasgow, Glasgow, G12 8QQ, UK.
¹⁰ Cholangiocarcinoma Screening and Care Program, (CASCAP) Khon Kaen University, Khon Kaen, 40002, Thailand.
¹¹ Institute of Zoology 1: Ecology and Parasitology, Karlsruhe Institute of Technology, KornblumenStrasse 13, Karlsruhe, Germany.
¹² Centre for Tropical Diversity and Climate change, James Cook University, Townsville, Australia.
¹³ Department of Environmental and Occupational Health, Colorado School of Public Health, 13001 E. 17th Place, B119, Aurora, CO, 8004510, USA.
¹⁴ Environmental Health Sciences, School of Public Health, University of California, Berkeley, CA, USA.
¹⁵ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. paib_sit@kku.ac.th.
¹⁶ Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. paib_sit@kku.ac.th.

PMID: 28238124
PMCID: PMC5756626
DOI: 10.1007/s00436-017-5401-6

Abstract

Opisthorchis viverrini is a major public health concern in Southeast Asia. Various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. We used four microsatellite loci to analyze O. viverrini adult worms originating from six species of cyprinid fish in Thailand and Lao PDR. Two distinct O. viverrini populations were observed. In Ban Phai, Thailand, only one subgroup occurred, hosted by two different fish species. Both subgroups occurred in fish from That Luang, Lao PDR, but were represented to very different degrees among the fish hosts there. Our data suggest that, although geographical separation is more important than fish host specificity in influencing genetic structure, it is possible that two species of Opisthorchis, with little interbreeding, are present near Vientiane in Lao PDR.

Keywords: Cyprinid fish; Host factors; Microsatellite DNA; Opisthorchis viverrini; Population genetics.

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Conflict of interest statement

Conflict of interest The authors declare that they have no conflict of interest.

Figures

**Fig.1**
The localities in Thailand and the Lao PDR where cyprinid fish were collected for determination of *Opisthorchis viverrini*

**Fig. 2**
Analyses of the optimal value of K in Structure from Structure Harvester using the Evanno method to indicate that two is the optimal value of K

**Fig. 3**
Structure model-based clustering showing segregation of *Opisthorchis viverrini* samples into two main subgroups (K = 2). In Ban Phai, Thailand, only one subgroup occurred, hosted by *C. apogon* and *H. dispar*. Both subgroups occurred in fish from That Luang, Lao PDR, but were represented to very different degrees among the fish hosts

See this image and copyright information in PMC

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Preliminary genetic evidence of two different populations of Opisthorchis viverrini in Lao PDR

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Preliminary genetic evidence of two different populations of Opisthorchis viverrini in Lao PDR

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