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. 1987 Jun;13(6):773-6.

[Site-specific endonuclease CauB31 from Chloroflexus aurantiacus B3]

[Article in Russian]
V M Kramarov, A I Fomenkov, N I Matvienko, R Kh Ubieta, V V Smolianinov
  • PMID: 2823833

[Site-specific endonuclease CauB31 from Chloroflexus aurantiacus B3]

[Article in Russian]
V M Kramarov et al. Bioorg Khim. 1987 Jun.

Abstract

A sequence-specific endonuclease CauB3I has been isolated from cell extracts of Chloroflexus aurantiacus and partially purified by chromatography on heparin-sepharose; the yield was 3000 units per 1 g of cells. The final preparation is free of non-specific nucleases. It is shown that endonuclease CauB3I recognizes 5' T decreases CCGGA 3' sequence in double-stranded DNA and cleaves it as shown by an arrow. Methylation of adenine in the recognition sequence makes it resistant to CauB3I.

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