[Effective method of introducing plasmid DNA into eukaryotic cells by using liposomes for producing vaccinia virus recombinants]
[Article in
Russian]
- PMID: 2823928
Item in Clipboard
[Effective method of introducing plasmid DNA into eukaryotic cells by using liposomes for producing vaccinia virus recombinants]
[Article in
Russian]
Biull Eksp Biol Med.
1987 Oct.
Abstract
The method of transfection of eukaryotic cells by plasmid DNA and production of recombinant vaccinia virus is described. The method is based on DNA encapsulation into reverse evaporation vesicles containing gangliosides, with a subsequent incubation of liposomes with vaccinia-infected cells in the presence of UV-inactivated Sendai virus. The recombinant DNA of vaccinia virus containing part of env gene of HTLV-3/LAV virus was obtained.
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