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. 2016;20(6):458-462.
doi: 10.5114/wo.2016.65605. Epub 2017 Jan 12.

Increased transforming growth factor β and interleukin 10 transcripts in peripheral blood mononuclear cells of colorectal cancer patients

Noyko S Stanilov  1 , Lyuba Miteva  1 , Geo Cirovski  2 , Spaska A Stanilova  1
Affiliations

Increased transforming growth factor β and interleukin 10 transcripts in peripheral blood mononuclear cells of colorectal cancer patients

Noyko S Stanilov et al. Contemp Oncol (Pozn). 2016.

Abstract

Aim of the study: The ability of immune cells in peripheral blood to produce certain cytokines affects tumour-elicited inflammation. The aim of this study was to investigate the gene expression of interleukin 12A (IL-12A), IL-12B, IL-23A, IL-10, IL-6, transforming growth factor β (TGF-β), HDAC3, and iNOS in peripheral blood mononuclear cells (PBMC) from colorectal cancer (CRC) patients.

Material and methods: The venous blood for PBMC isolation was collected preoperatively and 10 days after surgery, from CRC patients. After isolation of total RNA and synthesis of cDNA, quantitative real-time PCR assays were performed.

Results: Our results demonstrated that among investigated cytokine genes IL-10 and TGF-β were significantly upregulated in patients with CRC compared to the control group, while the expression of IL-23 mRNA was significantly decreased in CRC patients. We observed significantly increased mRNA levels in CRC patients' PBMC before surgery for IL-10 and TGF-β compared to both postoperative and control groups. We also found a significant upregulation of iNOS in early compared to advanced CRC.

Conclusions: Based on the results we can assume that PBMC gene expression programming in CRC patients drives local differentiation of Th cells towards Treg instead of the Th1 anti-tumour subpopulation.

Keywords: CRC; HDAC3; Treg; cytokine; iNOS; mRNA; qPCR.

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Figures

Fig. 1
Fig. 1
Relative quantity of cytokine gene expression in PBMC before and 10 days after surgery of CRC patients and healthy controls
Fig. 2
Fig. 2
Relative quantity of cytokine gene expression in pre-operative PBMC of patients with early and advanced of CRC and healthy controls
See this image and copyright information in PMC

References

    1. Cruz-Bustillo CD. Molecular genetics of colorectal cancer. Rev Esp Enferm Dig. 2004;96:48–9. - PubMed
    1. Hanahan D, Weinberg R. Hallmarks of Cancer: The Next Generation. Cell. 2011;144:646–74. - PubMed
    1. Balkwill F, Mantovani A. Inflammation and cancer: back to Virchow? Lancet. 2001;357:539–45. - PubMed
    1. Colotta F, Allavena P, Sica A, Garlanda C, Mantovani A. Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability. Carcinogenesis. 2009;30:1073–81. - PubMed
    1. Demaria S, Pikarsky E, Karin M, et al. Cancer and inflammation: promise for biologic therapy. J Immunother. 2010;33:335–51. - PMC - PubMed