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. 2017 Apr 24;61(5):e01204-16.
doi: 10.1128/AAC.01204-16. Print 2017 May.

Colistin Resistance Gene mcr-1 and Its Variant in Escherichia coli Isolates from Chickens in China

Yong-Qiang Yang  1 , Yun-Xia Li  1 , Tao Song  2 , Yan-Xian Yang  1 , Wei Jiang  1 , An-Yun Zhang  1 , Xin-Yi Guo  3 , Bi-Hui Liu  1 , Yong-Xiang Wang  1 , Chang-Wei Lei  1 , Rong Xiang  1 , Hong-Ning Wang  4
Affiliations

Colistin Resistance Gene mcr-1 and Its Variant in Escherichia coli Isolates from Chickens in China

Yong-Qiang Yang et al. Antimicrob Agents Chemother. .

Abstract

The mcr-1 gene was detected in 5.11% (58/1136) of Escherichia coli isolates of chicken origin from 13 provinces in China. A novel mcr-1 variant, named mcr-1.3, encoding an Ile-to-Val functional variant of MCR-1 was identified in a sequence type 155 (ST155) strain. An mcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), was identified. The transfer of pHeN867 led to a 32-fold increase in the MIC of colistin in the recipient, exhibiting an effect on colistin resistance that was similar to that of mcr-1.

Keywords: E. coli; colistin resistance; mcr-1.3; plasmid.

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Figures

FIG 1
FIG 1
PFGE patterns of chromosomal DNA restriction fragments from 58 mcr-1-positive E. coli strains from chickens. CST, colistin; CTX, cefotaxime; AMC, amoxicillin-clavulanic acid; CAZ, ceftazidime; IPM, imipenem; CIP, ciprofloxacin; LVX, levofloxacin; NAL, nalidixic acid; AMK, amikacin; GEN, gentamicin; DOX, doxycycline; SXT, trimethoprim-sulfamethoxazole; STR, streptomycin; ATM, aztreonam; PIP, piperacillin; ST, sequence type.
FIG 2
FIG 2
Plasmid structures of pHeN867, pHNSHP45, and pAF23. Light blue shading denotes shared regions of homology. ORFs are indicated by arrows and colored based on their predicted gene functions.
See this image and copyright information in PMC

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