A Bacillus subtilis phage phi 29 transcription terminator is efficiently recognized in Streptomyces lividans

Abstract

A DNA fragment from the Bacillus subtilis phage phi 29, containing the bidirectional transcription terminator TD1, where the right early transcription and late viral transcription terminate, has been inserted in one orientation between the aminoglycoside phosphotransferase (APH) gene (neo) and the phi 29 main early and late promoters present in derivative constructs of the Streptomyces promoter-probe plasmid pIJ486. The TD1 terminator is efficiently recognized in S. lividans and ends the transcription, started in vivo at the phi 29 promoters, at the same point as the B. subtilis RNA polymerase, resulting in a considerable reduction of the level of the APHII enzyme synthesized under the control of the phage promoters.