Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Nov;77(3):259-62.
doi: 10.1007/BF00284481.

Genes for two homologous G-protein alpha subunits map to different human chromosomes

E J Neer  1 , T Michel, R Eddy, T Shows, J G Seidman
Affiliations

Genes for two homologous G-protein alpha subunits map to different human chromosomes

E J Neer et al. Hum Genet. 1987 Nov.

Abstract

Signal transduction across biological membranes is modulated by a family of related GTP-binding proteins termed G proteins. These G proteins have a heterotrimeric structure composed of alpha, beta, and gamma subunits. The alpha subunits of the G proteins bind GTP and appear to determine the biochemical specificity of the protein. We have recently cloned and characterized cDNA encoding two G-protein alpha subunits, alpha i and alpha h. The former is a substrate for ADP-ribosylation by pertussis toxin. The protein corresponding to alpha h has not yet been identified. These cDNAs encode proteins, which demonstrate 90% sequence identity to one another and also show marked similarity to other G proteins. The present studies were designed to determine whether the genes for these related proteins are clustered on a single human chromosome. Genomic DNA isolated from a panel of mouse-human hybrid cell lines was analyzed by hybridization to cDNAs for alpha i and alpha h. Based on the distribution patterns of alpha i and alpha h in cell hybrids, the gene for alpha i was assigned to human chromosome 7, and the gene for alpha h assigned to chromosome 12. These data suggest that the G-protein gene family may be distributed over at least two human chromosomes.

PubMed Disclaimer

References

    1. Bray P, Carter A, Simons C, Guo V, Puckett C, Kamholz J, Spiegel A, Nirenberg M. Human cDNA clones for four species of Gαs signal trasduction protein. Proc Natl Acad Sci USA. 1986;83:8893–8897. - PMC - PubMed
    1. Fong HKW, Hurley JB, Hopkins RS, Miake-Lye R, Johnson MS, Doolittle RF, Simon MI. Repetitive segmental structure of the transducin β subunit: homology with the CDC4 gene and identification of related mRNAs. Proc Natl Acad Sci USA. 1986;83:2162–2166. - PMC - PubMed
    1. Gill DM, Meren R. ADP-ribosylation of membrane proteins catalyzed by cholera toxin: basis of the activation of adenylate cyclase. Proc Natl Acad Sci USA. 1978;75:3050–3054. - PMC - PubMed
    1. Itoh H, Kozasa T, Nagata S, Nakamura S, Katada T, Ui M, Iwai S, Ohtsuka E, Kawasaki H, Suzuki K, Kaziro Y. Molecular cloning and sequence determination of cDNAs for α subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. Proc Natl Acad Sci USA. 1986;83:3776–3780. - PMC - PubMed
    1. Lerea CL, Somers DE, Hurley JB, Klock IB, Bunt-Milan AH. Identification of specific transducin α subunits in retinal rod and cone photoreceptors. Science. 1986;234:77–80. - PubMed

Publication types

LinkOut - more resources