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. 2017 Jan 25;9(1):3592-3597.
doi: 10.19082/3592. eCollection 2017 Jan.

Investigation of the Best Saccharomyces cerevisiae Growth Condition

Roshanak Salari  1 , Rosita Salari  2
Affiliations

Investigation of the Best Saccharomyces cerevisiae Growth Condition

Roshanak Salari et al. Electron Physician. .

Abstract

Introduction: Saccharomyces cerevisiae is known as one of the useful yeasts which are utilized in baking and other industries. It can be easily cultured at an economic price. Today the introduction of safe and efficient carriers is being considered. Due to its generally round shape, and the volume that is enclosed by its membrane and cell wall, it is used to encapsulate active materials to protect them from degradation or to introduce a sustained release drug delivery system. Providing the best conditions in order to achieve the best morphological properties of Saccharomyces cerevisiae as a carrier.

Methods: In this research, the most suitable growth condition of yeast cells which provides the best size for use as drug carriers was found by a bioreactor in a synthetic culture medium. Yeast cell reproduction and growth curves were obtained, based on pour plate colony counting data and UV/Visible sample absorption at 600 nm. Yeast cell growth patterns and growth rates were determined by Matlab mathematical software.

Results: Results showed that pH=4 and dissolving oxygen (DO) 5% was the best condition for yeast cells to grow and reproduce. This condition also provided the largest size (2 × 3 μ) yeast cells.

Conclusion: Owing to the yeast cells' low-cost production and their structural characteristics, they could be used as potent drug carriers.

Funding: This work was supported by a grant from the Vice Chancellor of Research of Mashhad University of Medical Sciences.

Keywords: Carrier; Growth; Mathematics; Saccharomyces cerevisiae yeast cells.

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Conflict of interest statement

Conflict of Interest: There is no conflict of interest to be declared.

Figures

Figure 1
Figure 1
Growth curves of Saccharomyces cerevisiae based on colony counting
Figure 2
Figure 2
Growth curves of Saccharomyces cerevisiae based on UV/Visible absorptions
Figure 3
Figure 3
Growth rate curve of Saccharomyces cerevisiae in pH=4 and DO5%
Figure 4
Figure 4
Light microscopy image of Saccharomyces cerevisiae in pH=4 and DO5%
Figure 5
Figure 5
SEM image of Saccharomyces cerevisiae in pH=4 and DO 5%
See this image and copyright information in PMC

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