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. 2017 Mar 20;56(6):3123-3126.
doi: 10.1021/acs.inorgchem.6b03108. Epub 2017 Feb 28.

Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake

Sarah R Nathan  1 , Nicholas W Pino  1 , Daniela M Arduino  2   3 , Fabiana Perocchi  2   3 , Samantha N MacMillan  1 , Justin J Wilson  1
Affiliations

Synthetic Methods for the Preparation of a Functional Analogue of Ru360, a Potent Inhibitor of Mitochondrial Calcium Uptake

Sarah R Nathan et al. Inorg Chem. .

Abstract

The mixed-valent oxo-bridged ruthenium complex [(HCO2)(NH3)4Ru(μ-O)Ru(NH3)4(O2CH)]3+, known as Ru360, is a selective inhibitor of mitochondrial calcium uptake. Although this compound is useful for studying the role of mitochondrial calcium in biological processes, its widespread availability is limited because of challenges in purification and characterization. Here, we describe our investigations of three different synthetic methods for the preparation of a functional analogue of this valuable compound. We demonstrate that this analogue, isolated from our procedures, exhibits potent mitochondrial calcium uptake inhibitory properties in permeabilized HeLa cells and in isolated mitochondria.

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