Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 May;35(5):438-440.
doi: 10.1038/nbt.3811. Epub 2017 Feb 27.

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

Yuan Zong  1   2 , Yanpeng Wang  1   2 , Chao Li  1   2 , Rui Zhang  1 , Kunling Chen  1 , Yidong Ran  3 , Jin-Long Qiu  4 , Daowen Wang  1 , Caixia Gao  1
Affiliations

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

Yuan Zong et al. Nat Biotechnol. 2017 May.

Erratum in

Abstract

Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.

PubMed Disclaimer

Comment in

  • Base editing on the rise.
    May A. May A. Nat Biotechnol. 2017 May 9;35(5):428-429. doi: 10.1038/nbt.3871. Nat Biotechnol. 2017. PMID: 28486457 No abstract available.

References

    1. Nat Methods. 2016 Dec;13(12 ):1029-1035 - PubMed
    1. Nat Biotechnol. 2013 Aug;31(8):686-8 - PubMed
    1. J Appl Genet. 2011 Nov;52(4):371-90 - PubMed
    1. Nat Methods. 2016 Dec;13(12 ):1036-1042 - PubMed
    1. J Genet Genomics. 2014 Feb 20;41(2):63-8 - PubMed

Publication types

MeSH terms