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. 2017 May;35(5):435-437.
doi: 10.1038/nbt.3816. Epub 2017 Feb 27.

Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim  1 , Seuk-Min Ryu  1   2 , Sang-Tae Kim  1 , Gayoung Baek  1 , Daesik Kim  2 , Kayeong Lim  1   2 , Eugene Chung  1   2 , Sunghyun Kim  1   2 , Jin-Soo Kim  1   2
Affiliations

Highly efficient RNA-guided base editing in mouse embryos

Kyoungmi Kim et al. Nat Biotechnol. 2017 May.

Abstract

Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations with an efficiency of 44-57% and allelic frequencies of up to 100%, demonstrating an efficient method to generate mice with targeted point mutations.

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Comment in

  • Base editing on the rise.
    May A. May A. Nat Biotechnol. 2017 May 9;35(5):428-429. doi: 10.1038/nbt.3871. Nat Biotechnol. 2017. PMID: 28486457 No abstract available.

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