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. 2017 Dec;55(1):1202-1206.
doi: 10.1080/13880209.2017.1282969.

Antimutagenic and anticlastogenic effects of Turkish Black Tea on TA98 and TA100 strains of Salmonella typhimurium (in vitro) and mice (in vivo)

Mohammad Charehsaz  1 , Hande Sipahi  1 , Ashok Kumar Giri  1 , Ahmet Aydin  1
Affiliations

Antimutagenic and anticlastogenic effects of Turkish Black Tea on TA98 and TA100 strains of Salmonella typhimurium (in vitro) and mice (in vivo)

Mohammad Charehsaz et al. Pharm Biol. 2017 Dec.

Abstract

Context: Black tea has been reported to have significant antimutagenic and anticarcinogenic properties associated with its polyphenols theaflavins (TF) and thearubigins (TR). Similarly, Turkish black tea (TBT) also contains a considerable amount of TF and TR.

Objective: This study investigated the mutagenic, antimutagenic and anticlastogenic properties of TBT.

Materials and methods: The mutagenic and antimutagenic effects of TBT (10 to 40000 μg/plate) were investigated in vitro on Salmonella strains TA98 and TA100 with and without S9 fraction. Anticlastogenic effect was studied at concentrations of 300-1200 mg/kg TBT extract by chromosomal aberrations (CA) assay from bone marrow of mice.

Results: The results of this study did not reveal any mutagenic properties of TBT. On the contrary, TBT extract exhibited antimutagenic activity at >1000 μg/plate concentrations in TA98 strain with and without S9 activation (40% inhibition with S9 and 27% without S9). In TA100 strain, the antimutagenic activity was observed at >20,000 μg/plate TBT extracts without S9 activation (28% inhibition) and at >1000 μg/plate with S9 activation (59% inhibition). A significant decrease in the percentage of aberrant cells (12.33% ± 1.27) was observed in dimethylbenz(a)anthracene (DMBA) plus highest concentration (1200 mg/kg) of TBT extract-treated group when compared to only DMBA-treated group (17.00% ± 2.28).

Discussion and conclusion: Results indicated that TBT can be considered as genotoxically safe, because it did not exert any mutagenic and clastogenic effects. As a result, TBT exhibited antimutagenic effects more apparently after metabolic activation in bacterial test system and had an anticlastogenic effect in mice.

Keywords: Ames test; Genotoxicity; chromosomal aberration assay.

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Figures

Figure 1.
Figure 1.
Antimutagenic activity (% inhibitory rate) of Turkish black tea extract against positive control (mutagen). 4-Nitro-o-phenylenediamine (NPD) (20 μg/plate) was used as positive control (mutagen) for S. typhimurium TA98 strain and sodium azide (SA) (1 μg/plate) was used as positive control (mutagen) for S. typhimurium TA100 without S9 activation. Benzo(a)pyrene (B(a)P) (1.0 μg plate) was used as positive control (mutagen) for both strains with S9 activation.
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