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Comment
. 2017 Feb 28;18(1):43.
doi: 10.1186/s13059-017-1179-1.

Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

Felix Wolter  1 , Holger Puchta  2
Affiliations
Comment

Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

Felix Wolter et al. Genome Biol. .

Abstract

Selection-free genome editing using Cas9 ribonucleoprotein embryo bombardment has been achieved for maize and wheat. This is a breakthrough that should make new breeding technologies more acceptable for worldwide use.

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Figures

Fig. 1
Fig. 1
Workflow used by Liang et al. [2] to achieve DNA-free editing of wheat genes. Cas9 is expressed in E. coli and purified. Single guide RNA (sgRNA) is transcribed in vitro and complexed with Cas9. This complex is coated onto 0.6 μm gold particles which are then bombarded into immature wheat embryo cells. Plants are regenerated without any selective agent from bombarded embryos and screened for mutations via PCR/restriction enzyme assay and sequencing
See this image and copyright information in PMC

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References

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