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. 1987 Dec;35(12):1457-60.
doi: 10.1177/35.12.2824601.

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method

C Y Li  1 , S C Ziesmer, O Lazcano-Villareal
Affiliations

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method

C Y Li et al. J Histochem Cytochem. 1987 Dec.

Abstract

We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.

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