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. 2017 Jan;10(1):29-33.
doi: 10.14202/vetworld.2017.29-33. Epub 2017 Jan 11.

Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh

Md Abdul Matin  1 , Md Ariful Islam  1 , Mst Minara Khatun  1
Affiliations

Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh

Md Abdul Matin et al. Vet World. 2017 Jan.

Abstract

Aim: This study was conducted for determination of the prevalence of colibacillosis in chicken in poultry farms in Mymensingh and Tangail districts. Isolation, identification, and antibiogram profile of Escherichia coli were also performed.

Materials and methods: A total of 25 chickens manifested clinical signs of colibacillosis were collected from five different poultry farms during natural outbreaks.

Results: In broiler, the prevalence of colibacillosis was 0.84%, and in layer, prevalence was 0.80%. The prevalence of colibacillosis was 1.0% and 0.5% in 25-30 days old and 31-35 days old broiler, respectively. In case of layer birds, the prevalence was 0.6% in 40-45 days old bird and 1% in 46-50 days old bird. Identity of the E. coli isolate of chicken was confirmed by sugar fermentation, biochemical tests, and polymerase chain reaction assay. Antibiogram profile of E. coli isolate of chicken revealed that it was multidrug resistant (resistant against two antibiotics, such as ampicillin and cefalexin).

Conclusion: Data of this study suggest that colibacillosis is prevalent in the study areas which underscore the need of implementation of prevention and control measure against this disease.

Keywords: Mymensingh; Tangail; chicken; colibacillosis; multidrug resistant Escherichia coli.

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Figures

Figure-1
Figure-1
Results of polymerase chain reaction for amplification of 16S rRNA of Escherichia coli isolates of chicken. Lane 1: 100 bp-12 kb size DNA marker (Trackit, Invitrogen, USA); 2: DNA of E. coli isolate from liver; 3: DNA of E. coli isolates from spleen; 4: DNA of E. coli isolate from feces; 5: Negative control without DNA.
Figure-2
Figure-2
Zone of inhibition of ampicillin, cefalexin, gentamicin, chloramphenicol and ciprofloxacin against Escherichia coli.
See this image and copyright information in PMC

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