Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:1556:223-236.
doi: 10.1007/978-1-4939-6771-1_11.

Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

Matthew Tierney  1   2 , Alessandra Sacco  3
Affiliations

Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

Matthew Tierney et al. Methods Mol Biol. 2017.

Abstract

Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.

Keywords: Engraftment; FACS-based cell isolation; Muscle regeneration; Skeletal muscle stem cells; Transplantation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Rando TA, Blau HM (1994) Primary mouse myoblast purification, characterization, and transplantation for cell-mediated gene therapy. J Cell Biol 125(6):1275–1287 - DOI - PubMed
    1. Bischoff R (1986) Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115(1):129–139 - DOI - PubMed
    1. Rosenblatt JD, Lunt AI, Parry DJ, Partridge TA (1995) Culturing satellite cells from living single muscle fiber explants. In Vitro Cell Dev Biol Anim 31(10):773–779 - DOI - PubMed
    1. Collins CA, Olsen I, Zammit PS, Heslop L, Petrie A, Partridge TA, Morgan JE (2005) Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. Cell 122(2):289–301 - DOI - PubMed
    1. Sherwood RI, Christensen JL, Conboy IM, Conboy MJ, Rando TA, Weissman IL, Wagers AJ (2004) Isolation of adult mouse myogenic progenitors: functional heterogeneity of cells within and engrafting skeletal muscle. Cell 119(4):543–554 - DOI - PubMed

Publication types

LinkOut - more resources