Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jan-Mar;9(1):74-79.
doi: 10.4103/0974-8490.199771.

Growth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil

P Rachitha  1 , K Krupashree  1 , G V Jayashree  1 , Natarajan Gopalan  2 , Farhath Khanum  1
Affiliations

Growth Inhibition and Morphological Alteration of Fusarium sporotrichioides by Mentha piperita Essential Oil

P Rachitha et al. Pharmacognosy Res. 2017 Jan-Mar.

Abstract

Objective: The aim of this study is to determine the phytochemical composition, antifungal activity of Mentha piperita essential oil (MPE) against Fusarium sporotrichioides.

Methods: The phytochemical composition was conducted by gas chromatography mass spectrometry (GC MS) analysis and mycelia growth inhibition was determined by minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), the morphological characterization was observed by scanning electron microscopy. Finally, the membrane permeability was determined by the release of extracellular constituents, pH, and total lipid content.

Result: In GC MS analysis, 22 metabolites were identified such as menthol, l menthone, pulegone, piperitone, caryophyllene, menthol acetate, etc. The antifungal activity against targeted pathogen, with MIC and MFC 500 μg/mL and 1000 μg/mL, respectively. The MPE altered the morphology of F. sporotrichoides hyphae with the loss of cytoplasm content and contorted the mycelia. The increasing concentration of MPE showed increase in membrane permeability of F. sporotrichoides as evidenced by the release of extracellular constituents and pH with the disruption of cell membrane indicating decrease in lipid content of F. sporotrichoides.

Conclusion: The observed results showed that MPE exhibited promising new antifungal agent against Fusarium sporotrichioides.

Summary: F. sporotrichioides, filamentous fungi contaminate to corn and corn--based productsF. sporotrichioides mainly responsible for the production of T-2 toxinPhytochemical composition was conducted by gas chromatography--mass spectrometry analysisMentha piperita essential oil (MPE) is commonly known as peppermintThe F. sporotrichioides growth was inhibited by MPE (minimum inhibitory concentration, minimum fungicidal concentration)Morphological observation by scanning electron microscope. Abbreviations Used: Cfu: Colony forming unit; DMSO: Dimethyl sulfoxide, °C: Degree celsius; F. Sporotrichoides: Fusarium sporotrichioides; EOs: Essential oils; M: Molar, g: Gram/gravity, mg: Milligram; μg: Microgram, ml: Milliliter; mm: Millimeter, min: Minutes; M. piperita: Mentha piperita, MIC: Minimum inhibitory concentration; MFC: Minimum fungicidal concentration; MAE: Mentha arvensis essential oil; Na2SO4: Sodium sulfate; pH: Potential Hydrogen; PDB: Potato Dextrose Broth; SEM: Scanning electron microscope.

Keywords: Fusarium sporotrichioides; Mentha piperita essential oil; gas chromatography-mass spectrometryGC-MS; scanning electron microscope.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Chromatogram of Mentha piperita essential oil
Figure 2
Figure 2
Inverted microscopic observation of Fusarium sporotrichioides: (a) Control, (b) fluconozole, (c) 500 μg/mL Mentha piperita essential oil, (d) 1000 μg/mL Mentha piperita essential oil
Figure 3
Figure 3
Scanning electron microphotography of Fusarium sporotrichioides: (a-c) control/untreated; (d-f) treated with Mentha piperita essential oil
Figure 4
Figure 4
Effects of the Mentha piperita essential oil on the 260 nm absorbing material release of Fusarium sporotrichioides ([◊]: control; [◼]: Minimum inhibitory concentration; [◂]: Minimum fungicidal concentration)
Figure 5
Figure 5
Effects of the Mentha piperita essential oil on the extracellular pH of Fusarium sporotrichioides ([▴]: Control; [◼]: Minimum inhibitory concentration; [◊]: Minimum fungicidal concentration)
None
Farhath Khanum
See this image and copyright information in PMC

References

    1. Edwards SG. Influence of agricultural practices on Fusarium infection of cereals and subsequent contamination of grain by trichothecene mycotoxins. Toxicol Lett. 2004;153:29–35. - PubMed
    1. Loogrieco A, Visconti A. On Overview on Toxigenic Fungi and Mycotoxins in Europe. The Netherlands: Kluwer Academic Publisher; 2004. pp. 1–251.
    1. Mielniczuk E, Kiecana I, Perkowski J. Susceptibility of oat genotypes to Fusarium crookwelense Burgess, Nelson and Toussoun infection and mycotoxin accumulation in kernels. Biol Bratisl. 2004;59:809–16.
    1. Brown DW, McCormick SP, Alexander NJ, Proctor RH, Desjardins AE. A genetic and biochemical approach to study trichothecene diversity in Fusarium sporotrichioides and Fusarium graminearum. Fungal Genet Biol. 2001;32:121–33. - PubMed
    1. Moss MO. Mycotoxin review – 2. Fusarium. Mycologist. 2002;16:156–60.

LinkOut - more resources