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. 2017 Jan;34(1):53-59.
doi: 10.4274/balkanmedj.2015.1411. Epub 2017 Jan 5.

Petroselinum Crispum is Effective in Reducing Stress-Induced Gastric Oxidative Damage

Ayşin Akıncı  1 , Mukaddes Eşrefoğlu  2 , Elif Taşlıdere  2 , Burhan Ateş  3
Affiliations

Petroselinum Crispum is Effective in Reducing Stress-Induced Gastric Oxidative Damage

Ayşin Akıncı et al. Balkan Med J. 2017 Jan.

Abstract

Background: Oxidative stress has been shown to play a principal role in the pathogenesis of stress-induced gastric injury. Parsley (Petroselinum crispum) contains many antioxidants such as flavanoids, carotenoids and ascorbic acid.

Aims: In this study, the histopathological and biochemical results of nutrition with a parsley-rich diet in terms of eliminating stress-induced oxidative gastric injury were evaluated.

Study design: Animal experimentation.

Methods: Forty male Wistar albino rats were divided into five groups: control, stress, stress + standard diet, stress + parsley-added diet and stress + lansoprazole (LPZ) groups. Subjects were exposed to 72 hours of fasting and later immobilized and exposed to the cold at +4 degrees for 8 hours to create a severe stress condition. Samples from the animals' stomachs were arranged for microscopic and biochemical examinations.

Results: Gastric mucosal injury was obvious in rats exposed to stress. The histopathologic damage score of the stress group (7.00±0.57) was higher than that of the control group (1.50±0.22) (p<0.05). Significant differences in histopathologic damage score were found between the stress and stress + parsley-added diet groups (p<0.05), the stress and stress + standard diet groups (p<0.05), and the stress and stress + LPZ groups (p<0.05). The mean tissue malondialdehyde levels of the stress + parsley-added group and the stress + LPZ group were lower than that of the stress group (p<0.05). Parsley supported the cellular antioxidant system by increasing the mean tissue glutathione level (53.31±9.50) and superoxide dismutase (15.18±1.05) and catalase (16.68±2.29) activities.

Conclusion: Oral administration of parsley is effective in reducing stress-induced gastric injury by supporting the cellular antioxidant defence system.

Keywords: Lansoprazole; gastric oxidative damages; parsley.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1. Macroscopic appearance of the inner gastric surface of a rat from the control group on the left and from the stress group on the right. Hyperaemia and haemorrhagic spots induced by stress are very obvious.
Figure 2
Figure 2. Stress group. Degeneration within the epithelium (E) and lamina propria was observed. HE; x20.
Figure 3
Figure 3. Stress + standard diet group. A weak staining is seen. PAS-AL; x40.
Figure 4
Figure 4. A. Stress + parsley-added diet group. Epithelial flattening (E) and degeneration are seen. HE; x20. B. Stress + parsley-added diet group. Positively stained thick mucus is observed on the surface. Violet colour in apical part of the glands (*) and blue colour (arrows) in the middle parts of the glands (arrows) staining was observed. PAS-Al; x20.
Figure 5
Figure 5. Stress + LPZ group. A normal histological appearance is seen. HE; x20.
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